Fig. 1
From: Age-related dysfunction of the DNA damage response in intestinal stem cells
The DDR is perturbed in old ISCs. Immunohistochemical analyses of DDR marker proteins were performed in young and old jejunum with (IR) or without 10 Gy X-ray irradiation (non-IR). Representative images stained with anti-pATR and anti-53BP1 antibodies, together with graphs of the percentages of positive cells, are shown in a and b, respectively. Arrowheads indicate GFP positive and pATR or 53BP1 negative cells. Data are mean ± s.e.m. (n = 3 and > 100 GFP+ cells). *p < 0.05, **p < 0.01, NS > 0.05. Scale bars, 50 μm