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Table 3 The total expression ratio of the gene of TGFβ1 in the MEFs treated with various concentrations of ethanol extract of Calendula (5 and 10 μg/ml) relative to control group is presented in each time (12 and 24 h after treatment). The statistic test for significance is randomization re-allocation test, implemented in the relative expression software tool. Significant down or upregulations of the genes highlighted

From: Calendula officinalis stimulate proliferation of mouse embryonic fibroblasts via expression of growth factors TGFβ1 and bFGF

  12 h after treatment 24 h after treatment
5 μg/ml 10 μg/ml 5 μg/ml 10 μg/ml
Relative expression 1.7 2.3 0.7 0.62
Standard error ± 0.21 ± 0.28 ± 0.24 ± 0.16
P value 0.235 0.001 0.490 0.359
Fold increase/decrease + 1.7 + 2.3 − 1.4 − 1.6