Table 4 The total expression ratio of the gene of TGFβ1 in the MEFs treated with various concentrations of methanol extract of Calendula (5 and 10 μg/ml) relative to control group is presented in each time (12 and 24 h after treatment). The statistic test for significance is randomization re-allocation test, implemented in the relative expression software tool. Significant down or upregulations of the genes highlighted
12 h after treatment | 24 h after treatment | |||
|---|---|---|---|---|
5 μg/ml | 10 μg/ml | 5 μg/ml | 10 μg/ml | |
Relative expression | 1.3 | 2.1 | 0.7 | 0.62 |
Standard error | ± 0.23 | ± 0.24 | ± 0.2 | ± 0.13 |
P value | 0.265 | 0.001 | 0.661 | 0.001 |
Fold increase/decrease | + 1.3 | + 2.1 | − 1.06 | − 2.5 |