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Table 5 The total expression ratio of the gene of bFGF in the MEFs treated with various concentration of ethanol extract of Calendula (5 and 10 μg/ml) relative to control group is presented in each time (12 and 24 h after treatment). The statistic test for significance is randomization re-allocation test, implemented in the relative expression software tool. Significant down or upregulations of the genes highlighted

From: Calendula officinalis stimulate proliferation of mouse embryonic fibroblasts via expression of growth factors TGFβ1 and bFGF

  12 h after treatment 24 h after treatment
5 μg/ml 10 μg/ml 5 μg/ml 10 μg/ml
Relative expression 1.9 2.2 0.42 0.39
Standard error ±0.24 ±0.29 ±0.19 ±0.18
P- value 0.256 0.001 0.001 0.001
Fold increase/decrease + 1.9 + 2.2 − 2.4 −2.5