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Table 6 The total expression ratio of the gene of bFGF in the MEFs treated with various concentration of methanol extract of Calendula (5 and 10 μg/ml) relative to control group is presented in each time (12 and 24 h after treatment). The statistic test for significance is randomization re-allocation test, implemented in the relative expression software tool. Significant down or upregulations of the genes highlighted

From: Calendula officinalis stimulate proliferation of mouse embryonic fibroblasts via expression of growth factors TGFβ1 and bFGF

  12 h after treatment 24 h after treatment
5 μg/ml 10 μg/ml 5 μg/ml 10 μg/ml
Relative expression 1.5 2.1 0.52 0.34
Standard error ± 0.25 ± 0.27 ± 0.17 ± 0.19
P value 0.231 0.001 0..275 0.001
Fold increase/decrease + 1.5 + 2.1 − 1.9 − 2.96