From: Review: corneal endothelial cell derivation methods from ES/iPS cells
Steps | EB culture | → | Co-culture with corneal stroma cells | |
Medium | DMEM/F12, 20% KSR, bFGF (8 ng/ml) etc. |  | FM: DMEM/F12, B27, EGF (20 ng/ml), bFGF (40 ng/ml), 10% FBS | EM: FM + LECCM (FM:LECCM = 3:1) |
Coating | Low adherence culture dish | Fibronectin, laminin, heparin sulfate-coated dish | ||
Duration | 7 days | 5 days | 2 weeks |