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Table 1 The summary of Zhang’s methods (2014). FM fibroblast differentiation medium, EM corneal endothelial cell differentiation medium, KSR knockout serum replacement, FBS fetal bovine serum, B27 B27 supplement, EB embryoid body

From: Review: corneal endothelial cell derivation methods from ES/iPS cells

Steps

EB culture

→

Co-culture with corneal stroma cells

Medium

DMEM/F12, 20% KSR, bFGF (8 ng/ml) etc.

 

FM: DMEM/F12, B27, EGF (20 ng/ml), bFGF (40 ng/ml), 10% FBS

EM: FM + LECCM (FM:LECCM = 3:1)

Coating

Low adherence culture dish

Fibronectin, laminin, heparin sulfate-coated dish

Duration

7 days

5 days

2 weeks