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Table 2 The summary of Chen’s methods (2015). IMDM; Iscove’s modified Dulbecco’s medium. N2; N2 supplement

From: Review: corneal endothelial cell derivation methods from ES/iPS cells

Steps EB culture LECCM culture
Medium IMDM, 15% FBS, etc. IMDM, 15% FBS, all-trans retinoic acid (1 μM), etc.   LECCM (DMEM/F12, N2, B27, bFGF (20 ng/ml), ascorbic acid, etc.)
Coating Low adherence culture dish Gelatin-coated dish
Duration 4 days 4 days 7 days