Table 2 The summary of Chen’s methods (2015). IMDM; Iscove’s modified Dulbecco’s medium. N2; N2 supplement
From: Review: corneal endothelial cell derivation methods from ES/iPS cells
Steps | EB culture | → | LECCM culture | |
Medium | IMDM, 15% FBS, etc. | IMDM, 15% FBS, all-trans retinoic acid (1 μM), etc. |  | LECCM (DMEM/F12, N2, B27, bFGF (20 ng/ml), ascorbic acid, etc.) |
Coating | Low adherence culture dish | Gelatin-coated dish | ||
Duration | 4 days | 4 days | 7 days | |