Table 2 Transplantation protocols
From: Toward the realization of cardiac regenerative medicine using pluripotent stem cells
Publication | Transplantation Method | Recipient Animal Species | Recipient Model | Donor Cell Type | Number of Donor Cells | Transplantation Efficacy |
|---|---|---|---|---|---|---|
Hattori F et al. [29] | Direct cell injection | Mouse | Healthy heart | mESC-CMs hESC-CMs | NA | NA |
Zwi-Dantsis L et al. [27] | Direct cell injection | Rat | Healthy heart | hiPSC-CMs | 1.5×105 | Develop gap junctions between donor hiPSC-CMs and host rat CMs |
Shiba et al. [28] | Direct cell injection | Pig | Cryoinjury induced infarcted heart | hESC-CMs | 1×108 | Reduce ventricular tachycardia Improve left ventricle fractional shortening |
Ye et al. [30] | Direct cell injection | Pig | LAD ligation induced infarcted heart | hiPSC-CMs hiPSC-EC hiPSC-SMC fibrin patch | 2×106 2×106 2×106 | Improve left ventricle ejection fraction and infarct size |
Chong JJ et al. [31] | Direct cell injection | Monkey | LAD balloon occlusion induced infarcted heart | hESC-CMs | NA | Electromechanical coupling between graft and host myocytes |
Shiba et al. [32] | Direct cell injection | Monkey | LAD ligation induced infarcted heart | hiPSC-CMs | 4×108 | Increase ventricular tachycardia transiently Improve left ventricle ejection fraction and fractional shortening |
Liu YW et al. [33] | Direct cell injection | Monkey | LAD balloon occlusion induced infarcted heart | hESC-CMs | 7.5×108 | Increase ventricular tachycardia transiently Improve left ventricle ejection fraction |
Tabei et al. [42] | Direct cell injection | Pig | Healthy heart | hiPSC-CM spheroids | 1×107 | The combination of the newly developed transplant device and spheroid formation promotes the retention of transplanted CMs |
Kimura et al. [34] | Pericardial endoscopy and direct injection | Pig | Healthy heart | NA | NA | NA |
Masumoto et al. [35] | Cell sheets | Rat | LAD ligation induced infarcted heart | mESC-CMs mESC-ECs&MCs | 5×105 5×105 | Improve left ventricle systolic function and infarct size |
Suzuki et al. [36] | Cell sheets Omentopexy | Rat | LAD ligation induced infarcted heart | Neonatal CMs | 5.6×105/cm2 | Improve left ventricle ejection fraction |
Kashiyama et al. [37] | Cell sheets | Monkey | LAD ligation induced infarcted heart | mkiPSC-CMs | 3.6×106/sheet | Improve left ventricle systolic function |
Ott HC et al. [38] | Cell scaffolding Recellularization | Rat | Decellularized heart | Neonatal CMs Fibrocytes ECs SMCs | 5-7.5×107 | Generate working recellularized heart |
Lu et al. [39] | Cell scaffolding Recellularization | Mouse | Decellularized heart | hiPSC-MCPs | 1×107 | Generate working recellularized heart having responsiveness to drugs |