Fig. 3
From: Osteochondrogenesis derived from synovial fibroblasts in inflammatory arthritis model
A subpopulation of SFs expressing Runx2, Sox9, and Col10a1 in pannus retain competence as OCs via HCs [16]. a, b In situ hybridization was performed. Sox9 (red), Runx2 (white), and Col10a1 (green) triple-positive synovial fibroblasts are present in pannus, along with Runx2 single-positive and triple-negative ones. c, d Immunohistochemical staining was performed. Osteocytes express ColI (green), and hypertrophic chondrocytes (HCs) express ColX (yellow). Both proteins are detected as extracellular matrix. In addition to pannus, both bone (osteocytes) and HCs express osterix (red). Each box in a and c represents a magnified enlargement area in each right panel. Scale bar = 20 μm (yellow) and 50 μm (white)