Fig. 5
From: Osteochondrogenesis derived from synovial fibroblasts in inflammatory arthritis model
Hypertrophic chondrocytes express Runx2, Sox9, and Col10a1. In situ hybridization was performed [16]. a, bSox9 (red), Runx2 (white), and Col10a1 (green) mRNA signals in hypertrophic chondrocytes (a) and osteocytes (b) in bColII-D1BC inflamed joints. c In pannus, OCs in bone, HCs, and synovial fibroblasts express podoplanin (green), vimentin (red), and S100A4 (yellow), respectively. Scale bar = 20 μm (yellow) and 50 μm (white). d, e Joint space narrowing, erosion, and osteoporosis in bColII-D1BC mice. D1BC and DBA/1J mice were injected with a low-dose of bColII to induce joint inflammation. Whole-mount bone and cartilage staining with alizarin red S and alcian blue, for bone (red) and cartilage (blue), respectively. All mouse limbs at 1 year after the first immunization. No ankylosis in CIA-induced DBA/1J mouse (d). Note, the complete destruction of all joints (ankylosis, allow) and shortening of the phalanges in bColII-D1BC mouse joints (e)