Fig. 3

The effect of CCG treatment during chemotherapy on AML cells. a Predicted activity of the top five upstream regulators based on the gene expression profiles of AML cells isolated from cytarabine-treated and untreated mice (21 days after AML cell transplantation). b Network analysis of the upstream regulators of MRTFA and its target genes. MRTFA activation led to SRF and MRTFB upregulation. c Kaplan–Meier plot showing survival of AML-injected mice treated with 200 μg CCG in 50 μL DMSO in addition to 20 μg cytarabine in 200 μL PBS. −CCG, n = 5; +CCG, n = 9; data from two experiments; **P < 0.0332 (log-rank test). d Representative intravital two-photon MIP skull images of AML control mice and AML CCG-treated mice. Green, EGFP-expressing AML cells; red, blood vessels (Alexa Fluor 594); blue, bone tissues (SHG). Scale bar, 50 μm. See also Video 3. e Representative images showing displacement area (left) and trajectories (right) of AML cells analyzed in (d). Scale bar, 50 μm. f, g Scatter plots showing displacement area ratio (f) and mean track speed (g) of all analyzed cells. The data were pooled from three or four mice per condition from independent experiments. −CCG, n = 80 (f), n = 119 (g); +CCG, n = 80 (f), n = 54 (g). Data are presented as mean ± SD. ****P < 0.0001 (Kolmogorov–Smirnov test)