Fig. 2

JQ1 decreases Brd4 occupancy at the Il34 gene promoter region. a Schematic representation of mouse Il34 gene locus. White and black boxes indicate untranslated region and coding domain sequence of Il34, respectively. R1 to 4 are possible promoter regions according to the Ensembl Genomes database. b Percent of input values of Brd4 in HM-1 cells analyzed by ChIP-qPCR. Genomic DNA from 10 μM of JQ1 or DMSO treated HM-1 cells was immunoprecipitated with anti-Brd4 antibody or control IgG and amplified by qPCR with Il34 each region’s primer pairs. Representative results as mean ± SEM (n = 3) from two independent expreriments. NS, not significant; **p < 0.01; Student’s t test. c Percent of input values of H3K27ac in HM-1 cells analyzed by ChIP-qPCR. Genomic DNA from 10 μM of JQ1 or DMSO treated HM-1 cells was immunoprecipitated with anti-H3K27ac antibody or control IgG and amplified by qPCR with Il34 each region’s primer pairs. Representative results as mean ± SEM (n = 3) from two independent expreriments. NS, not significant; **p < 0.01; Student’s t test. d Percent of input values of H3K4me3 in HM-1 cells analyzed by ChIP-qPCR. Genomic DNA from 10 μM of JQ1 or DMSO treated HM-1 cells was immunoprecipitated with anti-H3K4me3 antibody or control IgG and amplified by qPCR with Il34 each region’s primer pairs. Representative results as mean ± SEM (n = 3) from two independent expreriments. NS, not significant; *p < 0.05, **p < 0.01; Student’s t test