Fig. 3
From: Bromodomain-containing protein 4 regulates interleukin-34 expression in mouse ovarian cancer cells
The anti-tumor effect of JQ1 is at least partly mediated by IL-34 suppression. aIl34 mRNA expression in Il34WT HM-1 or Il34OE HM-1 treated with JQ1 (n = 3). Data represent mean ± SEM. NS, not significant; *p < 0.05; Student’s t test. b Experimental design of JQ1 treatment in vivo. Mice were inoculated with wild-type HM-1 cell line which spontaneously produces IL-34 (Il34WT HM-1) and with Il34-overexpressing cell line (Il34OE HM-1). Mice were treated with JQ1 or vehicle control for 3 weeks. c Tumor growth in B6C3F1 mice inoculated with Il34WT HM-1 or Il34OE HM-1 and treated with JQ1 or vehicle control. (control: n = 4, JQ1: n = 3). NS, not significant; *p < 0.05, **p < 0.01; Student’s t test, error bars represent SEM. d Macroscopic observation of subcutaneously injected tumors in B6C3F1 mice sacrificed on day 27. Scale bars 1 cm. e The expression level of Il34 normalized by Gapdh in HM-1 tumors collected on day 27 (control: n = 4, JQ1: n = 3). NS, not significant; *p < 0.05, **p < 0.01; Student’s t test. Error bars represent SEM