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Table 1 Fluorescent-labeled mice for intravital multiphoton imaging in bone

From: Intravital imaging of orthotopic and ectopic bone

Target gene/promoter

Fluorescence (color)

Labeled cells

Report

(development)

Report

intravital imaging)

CX3CR1

EGFP (green)

Macrophage

Jung et al. Mol. Cell. Biol. (2000) [16].

Ishii et al. Nature (2009) [17].

V-ATPase a3 subunit promoter

GFP (green)

Macrophage

Sun-Wada et al. J Cell Sci. (2009) [14].

Kikuta et al. J. Clin. Invest. (2013) [12].

TRAP (exon 2)

tdTomato (red)

Osteoclasts

Kikuta et al. J. Clin. Invest. (2013) [12].

Kikuta et al. J. Clin. Invest. (2013) [12]. Furuya et al. Nat. Commun. (2018) [13].

Rat COL1A1 promoter (2.3 kb)

ECFP (cyan)

Osteoblasts

Furuya et al. Nat. Commun. (2018) [13].

Furuya et al. Nat. Commun. (2018) [13]. Hashimoto et al. Sci Rep. (2020) [15].

Rat COL1A1 promoter (2.3 kb)

GFP (green)

Osteoblasts

Kalajzic et al. J. Bone Miner. Res. (2002) [18].

Kalajzic et al. J. Bone Miner. Res. (2002) [15].

Villa et al. Tissue Eng Part C Methods. (2013) [19].

Huang C, et al. J Bone Miner Res. (2015).

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  1. CX3CR1 C-X3-C motif chemokine receptor 1, EGFP enhanced green fluorescent protein, V-ATPase V-type H+-ATPase, GFP green fluorescent protein, TRAP tartrate-resistant acid phosphatase, COL1A1 collagen type 1 alpha 1, ECFP enhanced cyan fluorescent protein