Table 3 Other methods for bone tissue visualization in intravital multiphoton imaging

Noncentrosymmetric molecular organization (collagen etc.)

SHG (blue, etc.*)

Bone etc.

Freund et al. Biophys J (1986) [23].

Hashimoto et al. Sci Rep. (2020) [15].

Villa et al. Tissue Eng Part C Methods. (2013) [19].

Ca²⁺

Alizarin (red)

Bone (mineral)

O’Brien et al. J Biomech. (2002) [26].

Hashimoto et al. Sci Rep. (2020) [15]. Villa et al. Tissue Eng Part C Methods. (2013) [19].