Fig. 1
From: Ex vivo generation of platelet products from human iPS cells
The expansion and production stages of imMKCLs. imMKCLs are established from iPSC-derived hematopoietic progenitor cells by the lentiviral gene transfection of c-MYC, BMI1, and BCLXL. By removing doxycycline (Dox-OFF), imMKCLs can switch from self-renewal to maturation with platelet production. These cells are cryopreserved as a master cell stock, and clinical grade platelets can be produced by performing the manufacturing process after thawing according to good manufacturing practice (GMP) guidelines [15, 25]