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Fig. 3 | Inflammation and Regeneration

Fig. 3

From: Possible NK cell-mediated immune responses against iPSC-derived cells in allogeneic transplantation settings

Fig. 3

Cytotoxic activity mediated by NK cells in the homo-to-hetero transplantation setting. a Schematic illustration of the experimental design. iPSCs were established from an HLA-haplotype homozygous individual as a putative donor and from an HLA-haplotype heterozygous person who carried HLA-C1 and HLA-C2 epitopes as a putative recipient. As target cells, T cells or vascular endothelial cells (VEs) were differentiated from these iPSCs. As effector cells, NK cells collected from peripheral blood mononuclear cells of a putative recipient were used. After effector and target cells were co-cultured for 6 h (T cells) or 18 h (VEs) in different E:T ratio’s, the percentage of dead target cells were measured by Cr51-release assay. b Cytotoxicity assay of NK cells isolated from the putative recipient against HLA-homo-derived T cells. **p < 0.01, Student’s t test. c Cytotoxicity assay of NK cells isolated from the putative recipient against HLA-homo-derived VEs. *p < 0.05, ***p < 0.001, Student’s t test

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