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Fig. 4 | Inflammation and Regeneration

Fig. 4

From: Possible NK cell-mediated immune responses against iPSC-derived cells in allogeneic transplantation settings

Fig. 4

Ectopic expression of the HLA-C2 molecule in regenerated grafts suppresses NK cell alloreactivity. a Schematic illustration of the experimental design. HLA-homo-C1/C1 iPSCs were transduced to express type 2 HLA-C allotype that is identical to the putative recipient using a lentiviral system. As negative control, HLA-homo-C1/C1 iPSCs transduced with HLA-C1 gene were also produced. Regenerated T cells or VEs from these iPSCs were used as target cells. After effector and target cells were co-cultured for 6 h in different E:T ratio’s, the percentage of dead target cells were measured by Cr51-release assay. b Cytotoxic assay of NK cells isolated from the putative recipient against different iPSCs-derived T cells. *p < 0.05, **p < 0.01, ***p < 0.001, Student’s t test. c Cytotoxic assay of NK cells isolated from the putative recipient against different iPSCs-derived VEs. **p < 0.01, ***p < 0.001, Student’s t test

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