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Fig. 2 | Inflammation and Regeneration

Fig. 2

From: Single-cell and spatial analyses of cancer cells: toward elucidating the molecular mechanisms of clonal evolution and drug resistance acquisition

Fig. 2

Scheme of the study revealing the EGFR-TKI resistance mechanism. For scATAC-seq, we used 10x Genomics. Before barcoding, nuclei were isolated and processed with Tn5 transpose. The processing time for nuclei isolation was determined using microscope (a). To reveal the resistance mechanism, we first established an EGFR-TKI-resistant cell line (b). We then analyzed this cell line model using scRNA-seq (c) and scATAC-seq (d). Additionally, we conducted scRNA-seq with clinical samples to confirm that the candidate mechanism occurs in vitro and in vivo (e)

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Inflammation and Regeneration

ISSN: 1880-8190

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