Fig. 3

Overview of previous and current single-cell multiome analysis. In the previous version of the 10x chromium system, we could not use identical cells in both scRNA-seq and scATAC-seq (a). We acquired the dataset from an identical cell using the new system (b). Due to changes in sample preparation before barcoding, nuclear RNA, not cellular RNA, was analyzed in the new system. c Each gel bead has two types of primers to identify each cell (left): primers for scRNA-seq (middle) and primers for scATAC-seq (right) (c). Using 10x barcodes, we could distinguish cells in both the scATAC-seq dataset and the scRNA-seq dataset