Fig. 1

Isolation and characterization of neural stem/progenitor cells (NS/PCs) from the subventricular zone (SVZ) of neonatal naked mole-rats (NMRs). a An adult NMR (left) and a NMR at postnatal day 1 (right). b Timeline of the experimental procedure for BrdU labeling. c Immunofluorescence images of SRY-box transcription factor 2 (SOX2; green), BrdU (magenta), and Hoechst 33342 (blue) in the neonatal NMR SVZ. Left, a low magnification image of Hoechst staining in the NMR neonatal brain. Center, an enlarged image of the boxed region in the left image. Right, a higher magnification image of the boxed region in the center image. White arrowheads indicate SOX2/BrdU double-positive cells. Scale bars: 500 μm (left), 500 μm (center), and 100 μm (right). LV, lateral ventricle. d Timeline of the experimental procedure for primary neurosphere and adherent culture of NS/PCs from the NMR SVZ. e Morphologies of NMR-NS/PCs under neurosphere and adherent culture conditions. The boxed regions in the left images are enlarged in the right images. Scale bars: 500 μm (left) and 100 μm (right). f Immunofluorescence images of SOX2, nestin (NES), and musashi RNA-binding protein 1 (MSI1) in NMR-NS/PCs under each culture condition. Blue, Hoechst 33342. Scale bars: 100 μm