Skip to main content
Fig. 4 | Inflammation and Regeneration

Fig. 4

From: Isolation and characterization of neural stem/progenitor cells in the subventricular zone of the naked mole-rat brain

Fig. 4

Comparison of the DNA damage response after γ-irradiation between neural stem/progenitor cells from naked mole-rats (NMR-NS/PCs) and mice (mouse-NS/PCs). a Immunofluorescence images of phosphorylated histone H2AX (γH2AX; magenta) and tumor protein p53-binding protein 1 (53BP1; green) in NMR- and mouse-NS/PCs exposed to 10 Gy of γ-radiation or left untreated. Blue, Hoechst 33342. White arrowheads indicate positive cells with >10 53BP1 foci per nuclei. Scale bars: 10 μm. b–d Quantification of the average signal intensity of γH2AX per nuclei (b), cells with >10 53BP1 foci (c), and cells with >10 phosphorylated ATM serine/threonine (pATM) foci (d). Data are mean ± standard deviation (SD) from n = 3 biological replicates. ***P < 0.001; ****P < 0.0001; N.S., not significant, two-way analysis of variance (ANOVA) followed by Bonferroni’s multiple comparisons test. e Quantification of cell death using propidium iodide (PI)/Hoechst staining after γ-irradiation. Data are mean ± SD from n = 3 biological replicates. ***P < 0.001; N.S., not significant, one-way ANOVA followed by Dunnett’s multiple comparison test. f Relative expression of genes downstream of p53 at 24 h after γ-irradiation determined by reverse transcription-quantitative PCR. Fold changes were calculated relative to the untreated control. Data are mean ± SD from n = 3 biological replicates. **P < 0.01; ***P < 0.001; ****P < 0.0001; N.S., not significant, unpaired t-test

Back to article page