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Fig. 2 | Inflammation and Regeneration

Fig. 2

From: Endothelial-specific depletion of TGF-β signaling affects lymphatic function

Fig. 2

Lymphatic vessels increase in TβRIIiΔLEC mice. a. Experimental schedule. Mice were treated with i.p. tamoxifen (40 mg/kg) for five consecutive days and sacrificed at day 22 (3 weeks). The ears shown using the dotted square and the tail shown using the dotted line were analyzed. b Whole-mount LYVE-1 staining of ear skin from control and TβRIIiΔLEC mice. Representable images are shown. Scale bar: 100 μm. c. Image-based analysis of LYVE-1-positive lymphatic vessel area. The average of LYVE-1-positive area in TβRIIiΔLEC mice (n = 4) was significantly higher than that in control mice (n = 4). d Number of branching points of lymphatic vessels. The average of the branching number in TβRIIiΔLEC mice (n = 4) was significantly higher than that in control mice (n = 4). e, f Immunohistostaining of the transverse section of the mice tails with anti-LYVE-1 (green) and anti-PECAM-1 (red) (e)/VE-Cadherin (red) antibodies (f). Sections were counterstained with DAPI (nucleus, blue). Scale bar: 100 μm. g Image-based analysis of LYVE-1-positive lymphatic vessel area of Fig. 2e from control (n = 3) and TβRIIiΔLEC mice (n = 3)

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