Skip to main content
Fig. 3 | Inflammation and Regeneration

Fig. 3

From: Endothelial-specific depletion of TGF-β signaling affects lymphatic function

Fig. 3

TβRIIiΔLEC mice exhibit decreased lymphatic drainage and tumor lymphatic metastasis. a. The mice were intraperitoneally administered Tx for five consecutive days. Three or 4 weeks later, LLC-GFP or FITC-dextran respectively were subcutaneously injected into their rear footpads independently. b Representative images of lymph flow from the lower extremities to the popliteal lymph node (PLN). (n = 7 mice per genotype) Scale bar: 5 mm. c Representative images of LLC-GFP metastasized to the PLN. Scale bar: 500 μm. d Quantification of metastasis (black) and no metastasis (white) to the PLN. Scale bar: 200 μm. (control; n = 7, TβRIIiΔLEC; n = 11) e Immunohistostaining of the sagittal section of PLN from control mice with anti-VEGFR3 (red) and anti-PECAM-1 (blue) antibodies. Green fluorescence was derived from LLC-GFP. Scale bar: 100 μm

Back to article page