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Fig. 5 | Inflammation and Regeneration

Fig. 5

From: Endothelial-specific depletion of TGF-β signaling affects lymphatic function

Fig. 5

LEC-specific TβRII deletion potentiates lymphangiogenesis. a–c Image-based analysis of PECAM-1-positive, VEGFR3-positive, or lymphatic lumen areas. There was no significant difference in the average of PECAM-1-positive area between TβRIIiΔLEC (n = 3) and control mice (n = 3) (a). The average of the VEGFR3-positive area (b) and the lymph lumen area surrounded by VEGFR3 (c) in TβRIIiΔLEC mice (n = 3) was significantly higher than that in control mice (n = 3). d Immunohistostaining of the tumor-derived sections used in Fig. 4 with anti-Ki-67 (green), and anti-PECAM-1 (red), and anti-VEGFR3 (purple) antibodies. Scale bar: 50 μm. e Image-based analysis of Ki-67-positive LECs (percentage of Ki-67+ cells/VEGFR3+ cells). f qPCR analysis of tumor tissue from control and TβRIIiΔLEC mice (n = 2 per genotype). Expression levels of VEGF-C gene are depicted as fold induction relative to the expression levels of control mice. The data presented are the means ± S.D. (n = 3)

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