Fig. 2

Therapeutic effects of ONO-1301 (8- or 28-week treatment) in Mc4r-KO NASH model mice. Mc4r-KO mice were fed a Western diet from 8 weeks of age and followed up for an additional 28 weeks. A Schematic of the experiment. B Serum levels of albumin (ALB), aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP), total bilirubin (T-Bil), total cholesterol (T-cho), and triglyceride (TG). Liver-to-body weight ratios were analyzed. C Sirius Red staining and D quantification of hydroxyproline levels. Total number of mice in each group: n = 10 in Ctl group, n = 8 in mid-ONO group, n = 6 in long-ONO group. Scale bar = 500 μm. Data are presented as mean ± standard deviation. ns: not significant