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Fig. 1 | Inflammation and Regeneration

Fig. 1

From: Generation of a BAC transgenic mouse strain that expresses CreERT and a fluorescent protein under the transcriptional control of the Fzd5 locus

Fig. 1

Generation of Fzd5-CreERT-tFP635 mice using BAC transgenic technology. A The BAC modification strategy to mark Fzd5-expressing cells by tamoxifen-inducible Cre recombinase (CreERT) and the fluorescent protein, TurboFP635 (tFP635). CreERT and three tandem copies of tFP635 cDNA were linked by P2A and inserted immediately after first ATG of the Fzd5 gene in BAC using a homologous recombination technique. B The copy numbers of BAC DNA in the Fzd5-CreERT-tFP635A, F, G, H, and I lines were assessed by quantitative genomic PCR in triplicate. C tFP635 expression in the femur from Fzd5-CreERT-tFP635H. The femur was sectioned according to Kawamoto’s method. After 4′,6-diamidino-2-phenylindole (DAPI) staining, sections were observed under a fluorescent microscope. TB trabecular bone, CB cortical bone

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