Fig. 2

Fzd5-CreERT-tFP635 marks LepR⁺MSC and some PαS in the long bones. The femurs and tibias were dissected from Fzd5-CreERT-tFP635I. A femur and tibia pair was used to prepare WB for PαS staining (A), while bone marrow (BM) was flushed out from the other pair for LepR⁺MSC staining (B). Frequencies of PαS, PDSP, SSP, and DN in tFP635⁺ non-hematopoietic cells (C) and that of LepR⁺MSCs in tFP635⁺ stromal cells (D) from the Fzd5-CreERT-tFP635A (n=4), F (n=7), G (n=4), H (n=4), and I line (n=6). E Frequencies of tFP635⁺ cells in PαS, PDSP, SSP, DN, and LepR⁺MSC from the Fzd5-CreERT-tFP635A (n=4), F (n=7), G (n=4), H (n=4), and I line (n=6). F Expression of Lepr and tFP635 in PαS and PDSP from Fzd5-CreERT-tFP635I. G Frequencies of Lepr⁺tFP635⁺ (DP), Lepr single-positive cells (Lepr⁺), tFP635 single-positive cells (tFP635⁺), and Lepr^-tFP635^- (DN) in PαS (Left panel) and PDSP (right panel) (n=3). H Frequencies of Lepr⁺ cells in tFP635⁺PαS and tFP635⁺PDSP (n=3). I RT-qPCR analysis of the indicated genes in tFP635^-PαS, tFP635⁺PαS, tFP635^-PDSP, and tFP635⁺PαS. Gapdh was used to normalize the amount of input RNA. All data are shown as the mean ± SEM. *P < .05, **P < .01, ***P < .005, ****P < .0005, and ****P < .00005 by the Student’s t test