Fig. 3

Tracing the lineage of Fzd5-expressing cells. A Strategy for the lineage tracing of Fzd5-expressing cells. B, C Fzd5-GFP were treated with TM at 8–12 weeks of age and the expression of GFP was analyzed in PαS (B) and LepR⁺MSC (C) at the indicated time points. D Time course changes in GFP positivity in PαS, PDSP, SSP, DN, LepR⁺MSC, EC, and HC are shown (n=5–10). The heat map shows p values against day 4. E Frequencies of GFP⁺ cells in APC, OPC, and Cd51⁺ stroma 16 weeks after the TM injection (n=9–11). G Expression of GFP and tFP635 in PαS from Fzd5-GFP 4 weeks after the TM injection (n=3). All data are shown as the mean ± SEM. *P < .05, **P < .01, ***P < .005, ****P < .0005, and ****P < .00005 by the Student’s t test