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Fig. 3 | Inflammation and Regeneration

Fig. 3

From: Single transcription factor efficiently leads human induced pluripotent stem cells to functional microglia

Fig. 3

High purity of microglial-like cells in hiMGLs derived by the PU protocol. A The purity of iMGLs was analyzed by flow cytometry at day 25 (also see Supplementary Fig 7). Whereas the marker of pan myeloid cells (CD45) was expressed equally by both hiMGLs and monocytic THP-1 population (more than 90%), microglia-specific markers (IBA1, CD11b, P2RY12, TMEM119, CX3CR1, PU.1, DAP12, and TREM2) were enriched in hiMGL compared to THP-1 (several thousand cells per each independent experiment). B Immunostaining of microglia-specific markers (IBA1, TMEM119, DAP12, CX3CR1, P2RY12, and TREM2) co-stained with PU.1 and Hoechst 33342 on hiMGLs at day 25. Scale bar, 75μm. C Western blot of DAP12 was performed using cell lysate of iPSC and PU-hiMGL. hiPSCs showed a low expression of DAP12 and its highly homologous DAP10 until they were differentiated into iMGLs. Both DAP12 and DAP10 were increased by 50% and 100% in cell lysates of hiMGL compared with those of hiPSC (n = 3 independent experiments)

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