Fig. 2

NLRP3 deficiency attenuated palmitic acid-induced impairment of SCs in vitro. A NLRP3 expression was detected in TM4 cells at 24 h after siNLRP3 infection (n = 6). B, C The IL-1β mRNA and protein expression in TM4 cells after PA incubation for 24 h (n = 6). D Caspase 1 activity in TM4 cells after PA incubation for 24 h (n = 6). E The protein expression of ZO-1 and occludin in TM4 cells after PA incubation for 24 h (n = 6). F Transepithelial electrical resistance was detected in TM4 cells after PA incubation for 48 h (n = 6). G The IL-1β mRNA in primary SCs isolated from NLRP3-deficient mice (n = 4–5). H Transepithelial electrical resistance was detected in primary SCs isolated from NLRP3-deficient mice (n = 4–5). I NLRP3 expression was detected in TM4 cells at 48 h after NLRP3 overexpression (n = 6). J The IL-1β mRNA and protein expression in TM4 cells at 48 h after NLRP3 overexpression (n = 6). K Caspase 1 activity in TM4 cells at 48 h after NLRP3 overexpression (n = 6). L, M The protein expression of ZO-1 and occludin in TM4 cells at 48 h after NLRP3 overexpression (n = 6). N Transepithelial electrical resistance was detected in TM4 cells at 48 h after NLRP3 overexpression (n = 6). Data are presented as the mean ± SD. For A and I–N, the statistical analysis was carried out by Student’s two-tailed t test; for others, the statistical analysis was carried out by one-way ANOVA. *P < 0.05 vs the matched control