Fig. 4
From: Diet-induced obesity impairs spermatogenesis: the critical role of NLRP3 in Sertoli cells
NLRP3 deficiency protected against HFD-induced subfertility in male mice. A Statistical results of the ratio of testis weight and tibia length in the indicated groups (n = 12). B, C HE staining of testes and tubular diameter from the indicated groups (n = 6). Black arrows indicate destruction of the BTB. D Sperm count, viability, and motility of the indicated groups (n = 6). E Serum testosterone concentration of the indicated groups (n = 6). F, G The mRNA of steroidogenic enzymes in the testes of obese mice (n = 6). H The expression of IL-1β in the testes of obese mice (n = 6). I Caspase 1 activity in the testes of obese mice (n = 6). J The nuclear accumulation of NF-κB was detected in the testes of obese mice (n = 6). K MMP-8 expression in the testes of obese mice (n = 6). L Immunofluorescence of occludin in the testes of obese mice (n = 5). M The protein expression of ZO-1 and occludin in the testes of obese mice (n = 6). Data are presented as the mean ± SD. Statistical analysis was carried out by one-way ANOVA. *P < 0.05 vs WT/ND; #P < 0.05 vs WT/HFD