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Fig. 6 | Inflammation and Regeneration

Fig. 6

From: Diet-induced obesity impairs spermatogenesis: the critical role of NLRP3 in Sertoli cells

Fig. 6

Constitutively active AMPKα attenuated palmitic acid-induced impairment of SCs in vitro. A, B The protein expression of AMPKα and ACC (n = 6). C The protein expression of p47phox in PA-treated TM4 cells at 24 h after caAMPKα infection (n = 6). D, E The production of ROS and NADPH oxidase activity in PA-treated TM4 cells at 24 h after caAMPKα infection (n = 6). FH The NLRP3 and IL-1β levels and caspase 1 activity in PA-treated TM4 cells at 24 h after caAMPKα infection (n = 6). I The nuclear NF-κB accumulation in PA-treated TM4 cells at 24 h after caAMPKα infection (n = 6). J, K MMP-8 expression in PA-treated TM4 cells at 24 h after caAMPKα infection (n = 6). L The protein expression of occludin in PA-treated TM4 cells at 24 h after caAMPKα infection (n = 6). Data are presented as the mean ± SD. For A, B, the statistical analysis was carried out by Student’s two-tailed t test; for others, the statistical analysis was carried out by one-way ANOVA. *P < 0.05 vs the matched control

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