Fig. 7

miR-451 inhibition suppressed palmitic acid-induced impairment of SCs in vitro. A, B The miR-451 level in HFD mouse testes (n = 6). C, D The miR-451 level in PA-treated mouse and human primary SCs (n = 6). E The luciferase assay in cultured TM4 cells (n = 6). F–J Cab mRNA and protein expression in cultured TM4 cells (n = 6). K The protein expression of AMPKα and ACC in PA-treated TM4 cells at 24 h after miR-451 inhibition (n = 6). L, M ROS production and NADPH oxidase activity in PA-treated TM4 cells at 24 h after miR-451 inhibition (n = 6). N The protein expression of NLRP3 and NF-κB in PA-treated TM4 cells at 24 h after miR-451 inhibition (n = 6). O–Q The IL-1β level, caspase 1 activity and MMP-8 in PA-treated TM4 cells at 24 h after miR-451 inhibition (n = 6). Data are presented as the mean ± SD. For A–E and G–J, the statistical analysis was carried out by Student’s two-tailed t test; for others, the statistical analysis was carried out by one-way ANOVA. *P < 0.05 vs the matched control