Fig. 4
From: Marine-derived microbes and molecules for drug discovery
Preparation of shark nanobodies. A Japanese bullhead shark. Nekozame in Japanese; Heterodontus japonicas. B Cloudy catshark. Torazame in Japanese; Scyliorhinus torazame. C Blood collection from a Japanese bullhead shark. Blood is drawn through a vascular sinus behind the dorsal fin using a sterile disposable needle and syringe. D Schematic representation of shark Ig new antigen receptor (IgNAR) and shark nanobody. Shark IgNAR consists of two identical protein chains each with one variable domain (VNAR) and five constant domains (C1NAR–C5NAR)