Fig. 3

Single-cell landscape of osteoclastogenesis. a Representative image of osteoclast differentiation culture system after 3 days of RANKL stimulation in bone marrow cells from CtsK-Cre CAG-CAT-EGFP mouse. The multinucleated giant cells labeled with EGFP are osteoclasts. Most of the cells in the culture system failed to differentiate into mature osteoclasts. Green (EGFP), CTSK; red, actin; blue, DAPI. b The osteoclast differentiation trajectory estimated by pseudotime analysis using the scRNA-seq data obtained from the in vitro osteoclast culture system. c Schematic of the stepwise cell fate decision pathways during osteoclastogenesis unveiled by scRNA-seq