Fig. 1

The miR-3129 gene located nearby osteoblast-specific SE domains and relevant eRNA expression. A Schematic diagram of the in silico analysis, based on the dbSUPER database. B Two osteoblast-specific SE (SE_46171 and SE_46098) domains are located near the miR-3129 gene on chromosome 2, and eight putative eRNAs may be expressed around H3K27ac. C, D Eight types of eRNA were highly expressed during the early stage of MSC differentiation into osteoblasts. The levels of eRNA expression were quantified by qPCR. The amounts of the eRNA transcripts were expressed relative to the amount of GAPDH transcript. Data are expressed as mean ± SD from three independent experiments (each n = 3 in C and D). ***p<0.001 vs. day 1 by one-way ANOVA. N. S, not significant; RQ, relative quantification