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Fig. 2 | Inflammation and Regeneration

Fig. 2

From: Enhancer RNA commits osteogenesis via microRNA-3129 expression in human bone marrow-derived mesenchymal stem cells

Fig. 2

miR-3129-5p expression was suppressed by SE inhibitors in a time-dependent and dose-dependent manner. A, C, E, G eRNA expression was examined at 24h after treatment of MSCs with the indicated concentrations of JQ1 (A) and THZ1 (E) and at the indicated time points after treatment of MSCs with 60 nM JQ1 (C) and 100 nM THZ1 (G). B, D, F, H Expression of miR-3129-5p and miR-23a was examined at 24h after treatment of MSCs with the indicated concentrations of JQ1 (B) and THZ1 (F) and at the indicated time points after treatment of MSCs with 60 nM JQ1 (D) and 100 nM THZ1 (H). The levels of eRNA and miRNA expression were quantified by qPCR. The amounts of eRNA and miRNA transcripts were expressed relative to the amounts of GAPDH and U6sn transcripts, respectively. Data are expressed as mean ± SD from three independent experiments (each n = 3 in A–H). ***p<0.001 vs. Veh or 0 h by one-way ANOVA. N.S, not significant; RQ, relative quantification; Veh, vehicle (DMSO)

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