Fig. 3

Knockdown of eRNA_2S suppressed miR-3129-5p expression in hBMSCs. A Positions of the putative eRNA_2, eRNA_4, and eRNA_7 transcripts during MSC differentiation into osteoblasts. B, D Each sense (B) and antisense (D) eRNA was examined in MSCs transfected with antisense LNA GapmeR. C, E miR-3129-5p expression was examined in MSCs transfected with sense (C) and antisense (E) LNA GapmeRs for 24h. The expression of miR-23a was examined as a control. The levels of eRNA and miRNA expression were quantified by qPCR. The amounts of eRNA and miRNA transcripts were expressed relative to the amounts of GAPDH and U6sn transcripts, respectively. Data are expressed as mean ± SD from three independent experiments (each n = 3 in B, C, D, and E). **p = 0.001, ***p < 0.001 vs. NC by Student’s t-test and one-way ANOVA. LNA, locked nucleic acid; NC, LNA GapmeR-negative control; S, sense; AS, antisense; RQ, relative quantification