Skip to main content
Fig. 2 | Inflammation and Regeneration

Fig. 2

From: IL-6 production through repression of UBASH3A gene via epigenetic dysregulation of super-enhancer in CD4+ T cells in rheumatoid arthritis

Fig. 2

eRNA promotes UBASH3A transcription in CD4+ T cells. A Genomic structure of UBASH3A, histone H3 containing the acetylated lysine27 (H3K27ac), and transcripts peaks. Putative eRNA_1-3 are expressed within the super-enhancer (SE_17452) in CD4+ T cells. H3K27ac are marked in GM12878 lymphoblastoid cells. RNA-sequencing data are shown in GM12878 and K562 leukemia cells. B Each antisense LNA GapmeR. LNA-eRNA denotes LNA needed for eRNA knockdown. C, D Jurkat cells were transfected with LNA-NC (lanes 1, 4, and 7), LNA-eRNA1_S (lane 2), LNA-eRNA1_AS (lane 3), LNA-eRNA2_S (lane 5), LNA-eRNA2_AS (lane 6), LNA-eRNA3_S (lane 8), and LNA-eRNA3_AS (lane 9). S and AS denote sense and antisense, respectively. The amount of each transcript was quantified by RT-qPCR and then expressed relative to that of GAPDH transcript. Data are mean ± standard deviation of three independent experiments. *P<0.05, vs control, by Dunnett’s multiple comparison test. RQ, relative quantification

Back to article page

Inflammation and Regeneration

ISSN: 1880-8190

Contact us