Fig. 3

Recruitment of BACH2 to UBASH3A locus in CD4⁺ T cells. A The UBASH3A locus displays super-enhancer architecture. Super-enhancer (SE_17452) domain mapped in CD4⁺ T cells is located at the UBASH3A locus. The locus is mapped to chromosome 21 at approximately 4,382,000–4,388,000. Histone H3 containing the acetylated lysine27 (H3K27ac) marks is provided in primary CD4⁺ T cells. BACH2-binding site is mapped to the indicated UBASH3A locus. ChIP assay was performed with the indicated antibodies across the UBASH3A loci in CD4⁺ T cells of healthy donors (n=5; HD_1, _2, _3, _4, and _5; male to female 1:4) and rheumatoid arthritis (RA) patients (n=5; RA_1, _2, _3, _4, and _5; male to female 1:4). DNA regions (1–3) were amplified by ChIP-PCR. B Results of ChIP-PCR. ChIP-PCR was performed as described under the “Methods” section. Five independent experiments were performed with similar results. The representative data are provided as shown in HD_1 versus RA_1 and HD_4 versus RA_4