Fig. 5

UBASH3A inhibits the production of IL-6 induced by TCR signaling in CD4⁺ T cells. A–D CD4⁺ T cells collected from healthy donors (HD) (A, B) and rheumatoid arthritis (RA) patients (C, D) were transfected with mock (lanes 1, 5, 9, and 13) and pcDNA3.1-UBASH3A (lanes 2, 6, 10, and 14) without CD3/CD28 stimulation for 24h, or mock (lanes 3, 7, 11, and 15) and pcDNA3.1-UBASH3A (lanes 4, 8, 12, and 16) with CD3/CD28 stimulation for 24h. After that, proinflammatory cytokine mRNA levels (A and C) were measured by RT-qPCR, and cytokine levels (B and D) were measured by cytometric bead array. E The relative mRNA expression levels of UBASH3A and IL-6 were analyzed in CD4⁺ T cells of the isolated PBMCs from patients with RA (n=24). Spearman’s test was used for the correlation analysis between two variables of interest. The amount of cytokines transcript was expressed relative to that of GAPDH transcript. Data are mean ± standard deviation from three independent experiments. *P<0.05, vs mock or mock+CD3/CD28, by Dunnett’s multiple comparison test. RQ, relative quantification