Fig. 1

An experimental SAH model by severity. a Simplified diagram of the experiment. In the SAH group, SAH was induced by blood injection 24 h before sacrifice. The neurocognitive score (modified Garcia score) was evaluated just prior to SAH induction and sacrifice (star). b Schematic diagram of the site of blood injection to create the subarachnoid hemorrhage model. c Photographs of the brains of each mouse after sacrifice showing no hematoma in the control and sham groups and increased hematoma volumes in the severe SAH group compared to the mild SAH group. d Intracranial pressure in each mouse during surgery. In the SAH groups, the hematoma was injected (red arrow) over 45 s, and the intracranial pressure gradually increased. The peak intracranial pressure was the highest in the severe SAH group. e Mortality of mice in the control, sham, and SAH groups (n = 18, total). f Modified Garcia score at 24 h is shown for each group. The severe SAH group had significant decreased neurological scores compared to those of the control group. Statistical analysis was performed with an unpaired two-tailed Student’s t test. The values in the bar graphs represent the mean ± SD. *p < 0.05