Fig. 10

Neural cell death in the posterior cerebral cortex (2.5 mm posterior to bregma). a Cells that were positive for both NeuN and TUNEL were quantified to assess neuronal cell death. The number of dead neural cells in layers I to III of the posterior region of the cerebrum was quantified (n = 3, each group). b Quantification of the number of dead neural cells in layers IV to VI of the posterior region of the cerebrum (n = 3, each group). c Quantification of the number of dead neural cells in all layers of the posterior region of the cerebrum (n = 3, each group). d Representative confocal images of TUNEL (green) and NeuN (red) in the posterior cortex. Nuclei were counterstained with Hoechst (blue). Scale bar, 50 μm. Statistical analyses were performed with one-way ANOVA and Tukey–Kramer post hoc tests. The values in the bar graphs represent the mean ± SD. *p < 0.05, NS: not significant