Fig. 6

Histological validation of human COL gene set. A Immunofluorescence images of Il18 (gray) of human colonic organoids in Matrigel (MG, top) and in collagen (COL, bottom) are shown. In MG panel, the region of organoids is indicated with a dashed while line. In the right panels, merged images with DAPI (blue) are shown. Scale bars, 50 μm. B Immunofluorescence images of IL18 (green) in surgical specimen from inflamed (inflammation) and non-inflamed (no inflammation) regions of ulcerative colitis are shown. Images are counterstained with DAPI (blue). The insets magnify areas indicated by a dashed square with staining of IL18 (gray) alone. A white dashed line in an enlarged gray image of non-inflamed region indicates a crypt/lamina propria boundary. Scale bar, 50μm. C Immunofluorescence images of KRT80 (gray) of human colonic organoids in Matrigel (MG, top) and in collagen (COL, bottom) are shown. In MG panel, the region of organoids is indicated with a dashed white line. In the right panels, colored images (green) counterstained with DAPI (blue) are shown. Scale bars, 50 μm. D Immunofluorescence images of KRT80 (green) in surgical specimen from inflamed (inflammation) and non-inflamed (no inflammation) regions of ulcerative colitis are shown. Images are counterstained with E-cadherin (red) and DAPI (blue). The insets magnify areas indicated by a dashed square with the staining of KRT80 (gray) and E-cadherin (gray) alone. Scale bar, 50μm