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Table 1 Culture conditions: culture conditions for mouse and human organoids are summarized in table

From: Collagen type I-mediated mechanotransduction controls epithelial cell fate conversion during intestinal inflammation

 

Species

Mouse

Human

Segments

Distal small intestine

Colon

Ages

Adult

Fetal

Adult

Medium

ENR

ENRWN

ENRWN

ENRWN

WRN CM

Recombinant

ECM

Matrigel

Matrigel

COL

Matrigel

Matrigel

COL

Murine EGF

50ng/ml

Mouse Noggin

100ng/ml

 

Mouse R-spo1

500ng/ml

 

Mouse Wnt3a

100ng/ml

 

   

300mg/ml

     

WRN-CM

20% v/v

    

 

Nicotinamide

10mM

 

 

N2

1% v/v

B27

2% v/v

BSA

1% v/v

 

  

Prostaglandin E2

2.5μM

     

NAC

1mM

    

 

Gastrin I

10nM

    

 

A83-01

500nM

    

 
  1. Abbreviations: BSA bovine serum albumin solution in 30% DPBS, CM conditioned medium, COL collagen type I, ECM extracellular matrix, R-spo1 R-spondin1