Fig. 1

Mononuclear cell infiltrates induced by ConA are decreased in Par2KO compared to WT mice. One day after injection, WT mice treated with ConA (10mg/kg) had areas of hemorrhage and mononuclear cell infiltrates (compare B, G to untreated mice in A, F). These infiltrates were less severe in the Par2KO (compare D, I to B, G), although specifically, hepatocellular necrosis is more apparent in Par2KO (D). In the WT, mononuclear cell infiltrates were more evident at day 14 (C, H), while in the Par2KO, there were no infiltrates (D, E, I, J). Scale bar = 75μm. K Quantification of mononuclear cell infiltrate in Par2-positive and negative areas. L Quantification of the hepatocellular damage (the percentage area of necrotic lesions) presented in A–J (n=4 for each condition). Note that hepatocellular damage is observed both in WT and Par2KO regardless of leukocyte infiltration. Black—statistical significance within the same group. Red—statistical significance in comparison to untreated WT. Blue—statistical significance between WT and KO at day 1. Statistical significance was measured using T test. *Indicates p<0.05, **p<0.01, ***p<0.005, error bars = SEM