Fig. 5

BM replacement experiment with high-dose ConA treatment show that the ConA effect is mediated by Par2 activation. A WT BM-reconstituted Par2KO mice 6 h after high-dose ConA injection showing hemorrhage. B Par2KO BM-reconstituted WT mice 6 h after high-dose ConA injection—hemorrhage was practically absent. Scale bar = 75μm. C Hemorrhage quantification (by hemosiderin area) shows that it appears in WT BM-reconstituted Par2KO mice and not in the reciprocal experiment (n=3 for each group). Statistical significance was measured using T test; error bars= SEM. D Kaplan-Meier survival plot of WT BM-reconstituted Par2KO mice (red, n=10) and Par2KO BM-reconstituted WT mice (blue, n=10) after 15mg/kg ConA injection. Log-rank (Mantel-Cox) test for p value = 0.0295. E–I Analysis of serum markers of liver damage 6 h after injection of 10 or 15 mg/kg ConA, determined by VetScan VS2. E Hemolysis (HEM). Note that 4 is the assay limit (#). F Alanine aminotransferase (ALT). G Bile acid (BA). H Total bilirubin (TBIL). I Gamma glutamyl transferase (GGT), (n=15, 3 for each group). Statistical significance was measured using T test. *Indicates p<0.05, **p<0.01, ***p<0.005, error bars = SEM. Black—between groups as indicated. Red—untreated WT control in comparison to all other groups or as indicated