Fig. 4

Treg and cytokine analysis. A Representative dot blots showing the CD4+ cell population in lymphocytes isolated from the spleen. B Representative dot blots showing the CD25+ cell population within the CD4+ cells. C Representative dot blots showing the FoxP3 cell population in the CD4+CD25+ cells. D Percentage of CD25+ Foxp3+Treg in the CD4+ lymphocytes isolated from the spleen. ^ap < 0.05 versus the C+2D-CM group, ^bp < 0.05 versus the C+2D-CM group, ^cp < 0.01 versus the C+3D-CM group, ^dp < 0.05 versus the C+2D-CM group, ^ep < 0.01 versus the D group, ^fp < 0.05 versus the C group, ^gp < 0.01 by the C+2D-CM group, ^hp < 0.01 versus the D group. E- Cytokine levels of the groups. ^ap<0.01 versus the C group (IL-4), ^bp < 0.05 versus the C group (IL-4), ^cp < 0.05 versus all groups (IL-10), ^dp < 0.05 versus the C group (IFNγ), ^ep < 0.05 versus the C+2D-CM group (IFNγ), ^fp < 0.01 versus the C group (IFNγ), ^gp < 0.001 versus the C+2D-CM group (IFNγ), ^hp < 0.05 by the C+3D-CM group (IFNγ). Data are presented as mean ± SEM (n = 8)