Skip to main content
Fig. 3 | Inflammation and Regeneration

Fig. 3

From: Next-generation proteomics of serum extracellular vesicles combined with single-cell RNA sequencing identifies MACROH2A1 associated with refractory COVID-19

Fig. 3

Enhanced expression of MACROH2A1 in monocytes of severely ill COVID-19 patients revealed by scRNA-seq of PBMCs. a Overview of single-cell analysis of PBMCs transcriptome obtained from patients with COVID-19 and healthy controls. b UMAP visualization of all 91,830 PBMCs from patients with COVID-19 and healthy controls. c Projection of MACROH2A1 gene expression. d Dot plots of MACROH2A1 expression for each cell of PBMCs. The color is scaled by average expression and the size of the dot is proportional to the percentage of the population expressing MACROH2A1. e Differential expression analysis of MACROH2A1 between indicated groups in each cell of PBMCs. f Differential expression analysis of MACROH2A1 between indicated groups in each subpopulation of monocytes. e,f The y-axis indicates expression changes of overall COVID-19 patient groups relative to healthy controls (blue), Groups 2 and 3 relative to healthy controls (red), Groups 2 and 3 relative to Group 1 (green), and Group 3 and Group 2 (purple), respectively. ncMono: non-classical monocytes, intMono: intermediate monocytes, cMono: classical monocytes, *p < 0.05, **p < 0.01, ***p < 0.001

Back to article page